Specific identification ofCandida albicans andCandida dubliniensis by PCR using species-specific primers
Makaleler > Tam Makale · 2008
Yazarlar: Özlem Abaci, Alev Halki-Uztan, Mustafa Ates
Annals of Microbiology, Uluslararası, Wos, 2008, cilt 58, ss 325-331
DOI: 10.1007/bf03175338
Özet
The aim of our study was to evaluate the effectiveness of phenotyping and genotyping methods for the identification ofCandida albicans andCandida dubliniensis. Phenotyping methods used are colony colour on CHROMagar Candida medium, growth at 45°C, TTC (2,3,5-triphenyl-tetrazolium chloride) reduction, germ tube and chlamydospores formation, and API 20C AUX. We also usedC. albicans andC. dubliniensis specific primers for identification performed with genotyping methods. DNAs ofC. albicans reference strains, differentCandida species,Cryptococcus neoformans, mycelial fungi, bacterial strains and human DNA were used as a template in order to evaluate the specificity of the primers. These primers yielded a 175-base-pair product only whenC. albicans DNA exists; however when other DNAs (except two isolates that are phenotypically identified asC. dubliniensis andC. tropicalis) exist no product appeared. In another PCR assay conducted by usingC. albicans DNA andC. dubliniensis specific primers, no product was observed.Candida albicans andC. dubliniensis specific primers were designed by Mannarelli and Kurtzman. Detection limit ofC. albicans primers was found to be 19 pg chromosomal DNA. In order to verify phenotypical definition with PCR amplification,C. albicans andC. dubliniensis specific primers were exposed to PCR by using DNAs that belong to 25 phenotypically defined oralCandida isolates.