Chitosan/Ionic Liquid Composite Electrode for Electrochemical Monitoring of the Surface‐Confined Interaction Between Mitomycin C and DNA
Makaleler > Tam Makale · 2013
Yazarlar: Ece Eksin, Mihrican Muti, Kadriye Arzum ERDEM GÜRSAN
Electroanalysis, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2013, cilt 25, ss 2321-2329
Özet
Abstract In the present study a chitosan/ionic liquid modified pencil graphite electrode (CHIT‐IL‐PGEs) was developed for the first time for enhanced electrochemical monitoring of nucleic acid, and the interaction of the anticancer drug Mitomycin C (MC) and calf thymus double stranded DNA (dsDNA) by measuring the oxidation signals of MC and guanine in the same voltammetric scale. Differential pulse voltammetry, cyclic voltammetry and electrochemical impedance spectroscopy techniques were used to evaluate the performance of the CHIT‐IL based biosensor on electrochemical monitoring of DNA, and drug‐DNA interaction. The experimental parameters, IL, dsDNA and MC concentration and the interaction time were then optimized.