LC-MS/MS measurement of leukocyte cystine; effect of preanalytic factors.
Makaleler > Tam Makale · 2020
Yazarlar: Erhan CANBAY, Ebru SEZER, Sema KALKAN UÇAR, M Çoker, Eser YILDIRIM SÖZMEN
Talanta, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2020, cilt 209, ss 120558-120558
DOI: 10.1016/j.talanta.2019.120558
Özet
Cystinosis is an autosomal recessive disorder characterized by the accumulation of cystine in lysosomes, causing irreversible damage to organs, especially the kidneys. Intracellular leukocyte cystine concentrations are used to diagnose cystinosis and to monitor cysteamine treatment. The aim of this study was to develop and validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method without derivatization capable of measuring leukocyte intracellular cystine concentrations.