Toxoplasma gondii RH Ankara: Production of evolving tachyzoites using a novel cell culture method
Articles > Article · 2011
Authors: Aysu DEĞİRMENCİ DÖŞKAYA, Mert DÖŞKAYA, Ayşe CANER, Candan ÇİÇEK, Metin KORKMAZ, Adnan Yüksel GÜRÜZ, AHMET ÜNER
Experimental Parasitology, Journal Indexed in SCI Expanded, International, WoS, 2011, vol 128, pp 1-8
DOI: 10.1016/j.exppara.2011.01.019
Summary
Toxoplasma gondii is one of the most researched parasite due to its easy growth both in vitro and in vivo. Tachyzoites, derived from mouse or rat peritoneum encounters ethical and economical problems when used for research or diagnostic purposes. Currently, research has focused on determining the most suitable cell culture environment to reach highest amount of viable tachyzoites with least host cell contamination. However, gene expression changes that take place throughout the adaptation of evolving T. gondii strains to continuous cell cultures appear as a problem.