Electrodeposition of carbon nanotubes and conjugation of arginyl-glycyl-aspartic acid for the following of glioblastoma cells on bionanocomposites
Makaleler > Tam Makale · 2025
Yazarlar: Belguzar Karadag, Simge Er Zeybekler, Sultan Sacide Gelen, Leila Sabour-Takanlou, Maryam Sabour-Takanlou, Çığır BİRAY AVCI, Dilek ODACI
BIOELECTROCHEMISTRY, SCI İndekslerine Giren Dergi, Uluslararası, Wos, 2025, cilt 164, ss 1-10
DOI: 10.1016/j.bioelechem.2025.108937
Özet
The improvement of surface treatment methods that permit the tuning of cell adhesion on the surface of biomaterials and devices is of considerable importance. Here, multi-walled carbon nanotubes (MWCNT) were modified with 4-aminothiophenol (4ATP). Then, electrodeposition of MWCNT-4ATP was carried out on 4ATPmodified screen-printed gold electrodes (SP-Au). After conjugation of Arginyl-glycyl-aspartic acid (RGD)-pep- tide on Poly(MWCNT-4ATP), the adhesion of U-87MG glioblastoma cells was examined by differential pulse voltammetry (DPV) technique. The synthesized MWCNT-4ATP and the obtained Poly(MWCNT-4ATP)/RGD surfaces were characterized using Scanning Electron Microscopy-Energy Dispersive X-Ray Spectrometer (SEMEDS), Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR-FTIR), X-Ray Photoelectron Spectrometer (XPS). The linear range for U-87MG glioblastoma cells was 102-106 cells/mL. The developed Poly (MWCNT-4ATP)/RGD cell adhesion platform provided monitoring of U-87MG glioblastoma cells using DPV technique and fluorescent imaging.