Carboxylated-Graphene Decorated Pencil Graphite Electrode as a Platform for Voltammetric Detection of DNA
Articles > Article · 2017
Authors: Erhan Zor, Ece EKŞİN, Mukerrem Findik, Haluk Bingol, Kadriye Arzum ERDEM GÜRSAN
Journal of The Electrochemical Society, Journal Indexed in SCI Expanded, International, WoS, 2017, vol 164, pp B723-B729
DOI: 10.1149/2.1971713jes
Summary
Continuous technology miniaturization using nanoscale materials allows the design of DNA sensing platforms with not only far larger surface areas but also more effective characteristics. We herein synthesized benzoic acid-functionalized graphene oxide (GOB), characterized by FT-IR, XPS, Raman Spectroscopy, TGA, SEM, TEM techniques and then it was utilized as a supporting material. Due to the benzoic acid groups available on the surface of GOB, fsDNA was directly immobilized onto the disposable pencil graphite electrode (PGE). The limit of detection (LOD) for fish sperm DNA (fsDNA) concentration was estimated to be 1.11 μg/mL by using differential pulse voltammetry (DPV) technique. It can be assumed that this simple modification protocol offers an alternative for the quick and robust monitoring of sequence-specific nucleic acids avoiding the need of complex or expensive requirements, and it can pave the way for new opportunities to design more novel DNA sensing strategies and applications on disposable platforms.